Analytical method, validation and degradation kinetics of Fluroxypyr- Meptyl in onion using gas chromatography-tandem mass spectrometry

A simple, fast and inexpensive analytical method was developed and validated for screening of Fluroxypyr-Meptyl, a selective post-emergent systemic broadleaf herbicide in onion (plant leaf, bulb) and soil using GC-MS/MS. The method comprised of extraction with ethyl acetate using matrix solid phase dispersion method followed by column chromatographic and dispersive solid phase extraction cleanup and quantified in GC-MS/MS. The method was validated in concentration ranging from 0.02-0.1 -1 μg g . The recovery of Fluroxypyr-Meptyl in onion (all substrates) and soil was ranging from 88-98% at different levels of -1 analytes with RSD (HorRat< 0.51) of 8-15%. The global uncertainty was calculated at 0.02 μg g . In order to evaluate in safety use in India and degradation kinetics, a field study was conducted following the validated method.